Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm. Organism i: Micromussa lordhowensis Imported. The chromophore was assumed to be neutral, and all titratable residues were taken in their standard … Enhanced cyan and yellow fluorescent proteins are widely used for dual color imaging and protein−protein interaction studies based on fluorescence resonance energy transfer. It has low acid sensitivity. ECFP is a basic (constitutively fluorescent) cyan fluorescent protein published in 1996, derived from Aequorea victoria. In addition to wild-type GFP and GFPuv proteins, we offer monomeric AcGFP1, which … However, yellow fluorescent protein presents some problems in that it is very sensitive to acidic pH and loses approximately 50 … Cyan fluorescent protein Imported Organism i: Acropora aculeus Imported.
Green fluorescent protein (GFP) was the first to be widely used for localizing proteins, but a number of variants with altered excitation and emission spectra, such as cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP), were quickly adopted to allow for colocalization studies. CFP is a basic (constitutively fluorescent) cyan fluorescent protein published in 1994, derived from Aequorea victoria. Among the important advances in the field have been the development of transgenic mice expressing various fluorescent proteins including green fluorescent protein (GFP) , red fluorescent protein (RFP) , and cyan fluorescent protein (CFP) . CFP, Cyan Fluorescent Protein: Source: E. coli: Appearance: Lyophilized protein: Physical Form Description: Freeze Dried: Molecular Weight: 31.3 kDa: Purity by SDS-PAGE ≥97%: Purity by ≥97%: Endotoxin Level: 0.1 ng/μg: Reconstitution Instructions: Reconstitute with dH₂O to 1 mg/ml: Handling: Centrifuge the vial prior to opening. Visualize and measure gene expression in a variety of organisms from bacteria to higher plants and animals with our selection of cyan and green fluorescent proteins. Cyan and green fluorescent proteins. Cyan fluorescent protein Imported Gene names i: Name:CFP Imported. 2006. Visualize and measure gene expression in a variety of organisms from bacteria to higher plants and animals with our selection of cyan and green fluorescent proteins. 1 Fluorescent Fusion Proteins. 400(3): p. 531-540. In addition to wild-type GFP and GFPuv proteins, we offer monomeric AcGFP1 , which is ideal for performing fusion studies. The two X-ray conformations A‘ and B‘ of ECFP were considered. The use of fluorescent proteins for in vivo imaging has opened many new areas of research . Enhanced yellow fluorescent protein is also useful for energy transfer experiments when paired with enhanced cyan fluorescent protein (ECFP) or GFP2. Cyan and yellow fluorescent proteins are efficient FRET donor and acceptors, and fusion of the two across a DEVD linker recognized by the executioner caspases allows measurement of both intact and cleaved molecules, as FRET efficiency (as measured by fluorescent emission ratios or by fluorescent lifetime) changes due to cleavage of the linker.

Biochem J. Use of these fluorescent proteins can be limited by their thermosensitivity, dim fluorescence, and tendency for aggregation. Hanna Fares, Alexander M van der Bliek, in Methods in Cell Biology, 2012. Ai HW, et al., Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence.
Cyan Fluorescent Protein. The dynamics and electronic absorption spectrum of enhanced cyan fluorescent protein (ECFP), a mutant of green fluorescent protein (GFP), have been studied by means of a 1 ns molecular dynamics (MD) simulation. Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. Here we report the results of a site-directed mutagenesis approach to improve these fluorescent …


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